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polyclonal goat anti mouse areg antibody  (R&D Systems)


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    Structured Review

    R&D Systems polyclonal goat anti mouse areg antibody
    Polyclonal Goat Anti Mouse Areg Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+goat+anti+mouse+areg+antibody/Goat+Anti-Mouse+IgG+Biotinylated+Antibody/bio_rxiv__64898__2026__01__28__701831-264-11-17
    Average 95 stars, based on 726 article reviews
    polyclonal goat anti mouse areg antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Immune cells adapt to distinct stem cell niches to govern tissue homeostasis
    Article Snippet: The following antibodies were used: CD45 AlexaFluor700 (30-F11, Biolegend, 1:200), TCR beta chain PerCP-Cy5.5 (H57-597, Biolegend, 1:200), TCRγδ BV421 or Alexa488 (GL3, Biolegend, 1:200), CD90.2 Alexa488 or PE-Cy7 (30-H12, Biolegend, 1:200), TCR Vγ5 APC or PE (536, Biolegend, 1:200), LAG-3 Alexa647 or PE-Cy7 (C9B7W, Biolegend, 1:200), IL-17 PE or PE-Cy7 (TC11-18H10.1, Biolegend, 1:200), IFNγ APC (XMG1.2, Biolegend, 1:200), CD4 PE-Cy7 (GK1.5, 1:200 Invitrogen), CD8 PE (53-6.7, 1:200, Biolegend), CXCR3 APC (CXCR3-173, Biolegend, 1:200), CD11b PE-Cy7 (M1/70, 1:200, Biolegend). .. For AREG intracellular cytokine staining, cells were incubated first with the polyclonal goat anti-mouse AREG antibody (1:200 RnD Systems), followed by donkey anti-goat Al488 or Al647 secondary antibody (1:300, Life Technologies). .. For intracellular cytokine staining, cells were incubated for 3h at 37°C in RPMI+10%FBS with 1μg/ml Ionomycin (BRAND), 100μg/ml PMA (BRAND) and BD Golgi plug (1:100, BD Biosciences).

    Incubation:

    Article Title: Immune cells adapt to distinct stem cell niches to govern tissue homeostasis
    Article Snippet: The following antibodies were used: CD45 AlexaFluor700 (30-F11, Biolegend, 1:200), TCR beta chain PerCP-Cy5.5 (H57-597, Biolegend, 1:200), TCRγδ BV421 or Alexa488 (GL3, Biolegend, 1:200), CD90.2 Alexa488 or PE-Cy7 (30-H12, Biolegend, 1:200), TCR Vγ5 APC or PE (536, Biolegend, 1:200), LAG-3 Alexa647 or PE-Cy7 (C9B7W, Biolegend, 1:200), IL-17 PE or PE-Cy7 (TC11-18H10.1, Biolegend, 1:200), IFNγ APC (XMG1.2, Biolegend, 1:200), CD4 PE-Cy7 (GK1.5, 1:200 Invitrogen), CD8 PE (53-6.7, 1:200, Biolegend), CXCR3 APC (CXCR3-173, Biolegend, 1:200), CD11b PE-Cy7 (M1/70, 1:200, Biolegend). .. For AREG intracellular cytokine staining, cells were incubated first with the polyclonal goat anti-mouse AREG antibody (1:200 RnD Systems), followed by donkey anti-goat Al488 or Al647 secondary antibody (1:300, Life Technologies). .. For intracellular cytokine staining, cells were incubated for 3h at 37°C in RPMI+10%FBS with 1μg/ml Ionomycin (BRAND), 100μg/ml PMA (BRAND) and BD Golgi plug (1:100, BD Biosciences).



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